Bios140 week 2 ilab – enzyme activity devry university

Bios140 week 2 ilab – enzyme activity devry university

BIOS140  Week 2 iLab – Enzyme Activity Devry University

Preview of solution:
NOTE: Save document as:  Lab2_FIRSTNAME_LASTNAME.docx, using your name in the filename. (This document is 4 pages long.)
Purpose: The purpose of this lab is to demonstrate how temperature, substrate concentration, and pH can impact the rate of an enzyme reaction. 

• In this experiment, you will use the Scientific Method to test your hypothesis.
• The enzyme used in this experiment is Invertase. This enzyme catalyzes the breakdown of sucrose (table sugar) into glucose and fructose. 
• Please contact me if you have any questions.

Results and Discussion:

Experiment A.  Impact of temperature on Invertase activity

1. Propose a hypothesis to predict the effect of an increase in temperature on Invertase activity.  (4 pts)
Since the temperature is increasing, the activity will also increase;  however, once it hits a certain temperature, it is going to decrease the activity.
Experiment Results (6 pts:  4 points for completed chart and 2 points for graph)

• After you record the experimental data in EnzymeLab, record the value of the slope of each reaction below. 

Experiment Run #	pH	Substrate concentration [S]	Temperature (°C)	Slope (Product Concentration vs. Temp)
1	7.0	90 mM	30	0.00081
2	7.0	90 mM	35	0.00152
3	7.0	90 mM	40	0.00226
4	7.0	90 mM	45	0.00282
5	7.0	90 mM	50	0.00300
6	7.0	90 mM	55	0.00298
7	7.0	90 mM	60	0.00274
8	7.0	90 mM	65	0.00244
9	7.0	90 mM	70	0.00211
10	7.0	90 mM	80	0.00136

INSERT GRAPH (V0 vs. Temp) HERE:

2. Temperature with maximum initial activity (Vo):    50   °C (1 pt) Based upon your hypothesis (above), is this what you expected? (1 pt)
 
My hypothesis was correct because 50°C  is the optimum temperature and from that point the activity will decrease. 
 
3. Do you think that Invertase isolated from any two organisms (for example, yeast Invertase vs. Invertase from the small intestine of humans) show the same temperature optimum? Why or why not? Explain your answer. (1 pt)
Even though yeast exist at a variety of temperature, he temperature optimum would be the same regardless of the source. 
4. Why do temperatures lower or higher than the optimum cause decreases in Invertase activity? (Be specific about how temperature is affecting the enzyme.) (1 pt)
Since Invertase is an enzyme, enzymes have limited ranges of effective temperatures because degradation of the chemical structure is at higher temperatures.  The enzyme might work at lower temperatures but it can take longer.  The reaction needs specific amount of energy to proceed and that is where obtaining the lower optimum temperature comes in.

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